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The cells were subsequently stained with Dil membrane dye (S0033, Beyotime Biotechnology, China) for 15 minutes and Hoechst 33342 nuclear dye (14533, Sigma-Aldrich, USA) for another 15 minutes after fixation with 4% paraformaldehyde

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(PubMed) A third mechanism is oxidative stress reduction

It is sharp and sometimes has uneven glass protruding